Hoch

Publikationen

Hier finden Sie alle wissenschaftlichen Publikationen und Arbeiten bzw. Veröffentlichungen von Dr. Gerald Böhm aus der Zeit von 1988 bis 2007. Die PDF-Dateien stehen zum Download gemäß den jeweiligen Urheberrechten bereit.

Proteins, independent of their mesophilic or thermophilic origin, consist exclusively of the 20 canonical natural amino acids. In the multicomponent system of the cytosol, these are known to undergo covalent modifications at the upper limit of temperature observed in the biosphere. Extremophiles must compensate for these degradation processes either by using compatible protectants or by enhanced synthesis and repair. Many studies on the thermal stability of proteins have focused on enzymes from hyperthermophiles. In this context, Thermotoga maritima, with its temperature range of growth between 55° and 90°, has become one of the favorite organisms, because it is widespread in marine geothermal vents as well as in low-salinity solfataric springs, it can be cultivated in large-scale fermentations, and it has been characterized in detail with respect to its metabolic requirements. The bacterium is a strictly anaerobic fermentative organotroph that grows on various sugars, cellulose, starch, and glycogen as the carbon source: peptides are required for growth on carbohydrates because the organism does not utilize ammonia or free amino acids as the N source.

Nach der Bereitstellung einer qualitativ hochwertigen Datenbank der humanen Genomsequenz und anderer wichtiger Genome wird der nächste große Schritt der Forschung in der Bestimmung wichtiger biologischer Funktionen der Bestandteile des zellulären Proteoms liegen. Hierzu kann die molekulare Bioinformatik mit ihrer anspruchsvollsten Disziplin, der Tertiärstrukturvorhersage von Proteinen anhand der Sequenz, wertvolle Hilfestellungen leisten. Die Entwickung neuartiger und automatisierbarer Verfahren zur Tertiärstrukturprognose im Rahmen der Vergleichenden Modellierung wird daher in Zukunft enorme Bedeutung gewinnen.

The development of novel delivery systems for therapeutic substances includes targeting of the carriers to a specific site or tissue within the body of the recipient. This can be accomplished by appropriate receptor-binding domains and requires linking of these domains to the carrier. We have used recombinantly expressed polyomavirus-like particles as a model system and inserted the sequence of a WW domain into different surface loops of the viral capsid protein VP1. In one variant, the WW domain maintained its highly selective binding properties of proline-rich ligands and showed an increased affinity but also an accelerated association/dissociation equilibrium compared to the isolated WW domain, thus allowing a short-term coupling of external ligands onto the surface of the virus-like particles.

 
 
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